Table 1 Primary antibodies used in the study
From: Airborne particulate matter (PM2.5) triggers ocular hypertension and glaucoma through pyroptosis
| Antibody | Source | Catalog No | Type of Ab | Dilution | MW |
|---|---|---|---|---|---|
| NLRP3 | Abcam | ab214185 | Rabbit polyclonal |
1:1000 (WB) 1:200 (IF) | 114 kD |
| Caspase-1 | Abcam | ab207802 | Rabbit polyclonal | 1:1000 (WB) | 35, 70 kD |
| Caspase-1 | Proteintech | 22,915–1-AP | Rabbit polyclonal | 1:200 (IF) | |
| IL-1β | Abcam | ab9722 | Rabbit polyclonal | 1:1000 (WB) | 30, 45 kD |
| GSDMD | Abcam | ab215203 | Rabbit monoclonal | 1:1000 (WB) | 35, 250 kD |
| GAPDH | Abcam | ab8245 | Mouse monoclonal | 1:1000 (WB) | 36 kD |